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dc.description.abstractLas interacciones del microbioma intestinal se han estudiado en una gran variedad de taxa. En casi todos los grupos, se ha encontrado una fuerte relación entre los microbios y sus huéspedes, pero los mecanismos evolutivos y ecológicos que determinan esas asociaciones no se han descrito completamente. Las hormigas (Hymenoptera: Formicidae) son un modelo interesante para explorar las implicaciones ecológicas y evolutivas en la estabilidad del microbioma. Proponemos una comparación del metagenoma de las hormigas de una variedad de especies que difieren en sus estrategias tróficas, preferencias de anidación, hábitat y relación filogenética. Nuestra hipótesis es que filogenia del hospedero será el factor principal que determine la composición de la comunidad microbiana entre las especies de hormigas, y el gradiente ambiental será el factor principal a nivel poblacional. Se recolectaron hormigas de diferentes hábitats en México y las principales subfamilias. Se disecaron gásteres de las hormigas y se extrajo el ADN. La región V4 del ARNr de SSU 16S bacteriano se amplificó en un secuenciador Illumina MiSeq. Se amplificaron las secuencias de COI de hormigas de las mismas colonias. Recuperamos 74 especies de hormigas diferentes de 334 nidos en 46 hábitats diferentes en México. Reportamos un nuevo registro en México y 19 nuevos registros (basados en el estado) de 14 especies. Describimos el metagenoma de 243 colonias que representan 7 subfamilias de hormigas y las comparamos en diferentes niveles taxonómicos. Discutimos la importancia de la historia evolutiva y el ambiente de la hormiga como factores de estabilización del microbioma y analizamos el microbioma entre diferentes poblaciones de especies de los géneros Camponotus, Pogonomyrmex y Atta. Proponemos que la filogenia es el factor que estabiliza el microbioma de 5 de las 7 subfamilias. Discutimos las implicaciones de la alta abundancia de pocos sOTUs de Wolbachia entre nuestras muestras.es_MX
dc.publisherUniversidad de Guanajuatoes_MX
dc.subject.classificationCGU- Doctorado en Ciencias (Biología) Tradicionales_MX
dc.titleEcological and evolutional implications of ant-microbiome associationses_MX
dc.subject.keywordsMolecular Microbial Ecologyen
dc.subject.keywordsEcología microbiana moleculares_MX
dc.contributor.oneMILAN JANDAes_MX
dc.description.abstractEnglishHost-microbial gut interactions have been studied in a large variety of taxa. In almost all groups, a strong relationship between microbes and their hosts has been found but the evolutive and ecological mechanisms that determine those associations haven’t been fully described. Ants (Hymenoptera: Formicidae) are an interesting model for exploring the ecological and evolutive implications in the microbiome stability. They are the dominant insect group in most terrestrial habitats and exhibit diverse life history strategies. They have a remarkable evolutionary strategy - eusociality, with overlapping generations within the colony, a cooperative brood care and a reproductive division of labor. The close relationship between individuals in the colony provides higher microbiome stability among the nestmates. The constant grooming in ants often prevents the growth of bacteria on the surface of individuals and influences the microbial communities inside the nest. Many ant species have glands that secrete a broad range of chemicals, usually with antimicrobial properties that enforce the aseptic conditions in the colony. We theorize that grooming, eusociality and gland products generate a highly selective pressure on the microbial communities associated with ants and could direct a highly specialized microbiome or core microbiome. Here we propose a comparison of the ants’ metagenome from a range of species that differ in their trophic strategies, nesting preferences, habitat and phylogenetic relationship. We hypothesize that the trophic strategies could be the principal factor that leads the microbial community composition among the ant ́s species, and the environmental gradient will be the main factor that influences the microbial community at the population level. Ants were collected from different habitats in México (deserts, tropical rain forest, cloud forests) and from all the main subfamilies. Gasters from seven individuals per nest have been dissected and DNA extracted using the power soil modified protocol, and the V4 region of the bacterial 16S SSU rRNA was amplified in an Illumina MiSeq sequencer. COI sequences from ants of the same colonies were also amplified. We retrieved 74 different ant species from 334 nests across 46 different habitats in México ranging from Cuatro Cienegas, Coahuila, to Bacalar, Chetumal. We report one new record in México and 19 new records (state-based) of 14 species. We describe the core metagenome of 243 colonies representing 7 ant subfamilies and we compare them at different taxonomic levels. We discuss the importance of the ant ́s evolutive history and environment as factors that could give stability to the core microbiome and we analyze the core microbiome among different populations of species from the genera Camponotus, Pogonomyrmex and Atta. We propose that at higher taxonomic levels the microbiome of 5 of the 7 subfamilies could be the principal factor for microbiome stability. Finally, we discuss the possible implications of the high abundance of few sOTUs of Wolbachia among our samples.en
Appears in Collections:Doctorado en Ciencias (Biología)

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