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http://repositorio.ugto.mx/handle/20.500.12059/3931Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.rights.license | http://creativecommons.org/licenses/by-nc-nd/4.0 | es_MX |
| dc.creator | Gabina Zeltzin Mendoza Lozano | - |
| dc.date.accessioned | 2021-02-05T19:32:29Z | - |
| dc.date.available | 2021-02-05T19:32:29Z | - |
| dc.date.issued | 2017 | - |
| dc.identifier.uri | http://repositorio.ugto.mx/handle/20.500.12059/3931 | - |
| dc.description.abstract | Se busca utilizar PCR en tiempo real como metodología para identificar la huella genética de cinco bacterias relacionadas al desarrollo de la enfermedad periodontal. Se utilizó ADN purificado de 5 bacterias ATCC Aggregatibacter actinomycetemcomitans43718,ATCCPorphyromonas gingivalis33277,ATCCPrevotella intermedia25611, ATCC Tannerella forsythia43037,ATCCTreponema denticola35405para amplificar un fragmento de 16S rRNA (para A.actinomycetemcomitans, P. Intermedia y T.forsythia) y waaA (para P. gingivalis y T. denticola) ,por qPCR utilizando química de Evagreen, logrando identificar molecularmente a las 5 bacterias. Finalmente se corrió en gel de agarosa para corroborar los fragmentos amplificados con los tamaños moleculares esperados. | es_MX |
| dc.language.iso | spa | es_MX |
| dc.publisher | Universidad de Guanajuato | es_MX |
| dc.relation | http://www.jovenesenlaciencia.ugto.mx/index.php/jovenesenlaciencia/article/view/1911 | - |
| dc.rights | info:eu-repo/semantics/openAccess | es_MX |
| dc.source | Jóvenes en la Ciencia: Verano de la Investigación Científica. Vol. 3, Num 2 (2017) | es_MX |
| dc.title | Huellas genéticas de bacterias de importancia en la salud bucal | es_MX |
| dc.type | info:eu-repo/semantics/article | es_MX |
| dc.subject.cti | info:eu-repo/classification/cti/3 | es_MX |
| dc.subject.keywords | Diagnóstico | es_MX |
| dc.subject.keywords | Molecular | es_MX |
| dc.subject.keywords | PCR en tiempo real | es_MX |
| dc.subject.keywords | Enfermedad Periodontal | es_MX |
| dc.type.version | info:eu-repo/semantics/publishedVersion | es_MX |
| dc.creator.two | Jorge Alejandro Alegría Torres | - |
| dc.creator.three | Nuria Patiño Marín | - |
| dc.creator.four | LIZETH GARCIA TORRES | - |
| dc.creator.idtwo | info:eu-repo/dai/mx/cvu/98061 | es_MX |
| dc.creator.idfour | info:eu-repo/dai/mx/cvu/490718 | es_MX |
| dc.description.abstractEnglish | The aim is to use real time PCR as a methodology to identify the genetic fingerprint of five bacteria related to the development of periodontal disease. Purified DNA from 5 bacteria ATCC Aggregatibacter actinomycetemcomitans 43718, ATCC Porphyromonas gingivalis 33277, ATCC Prevotella intermedia 25611, ATCC Tannerella forsythia 43037, ATCC Treponema denticola 35405 was used to amplify a fragment of 16S rRNA (for A.actinomycetemcomitans, P. Intermedia and T. Forsythia) and waaA (for P. gingivalis and T. denticola), by qPCR using chemistry of Evagreen, being able to identify molecularly the 5 bacteria. Finally it was run on agarose gel to corroborate the amplified fragments with the expected molecular sizes | - |
| Appears in Collections: | Revista Jóvenes en la Ciencia | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Huellas genéticas de bacterias de importancia en la salud bucal.pdf | 318.5 kB | Adobe PDF | View/Open |
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